Fig 2: RT-qPCR validation of the differentially expressed genes (DEGs) obtained by RNA sequencing (RNA-Seq). (A) The genes involved in inflammation. (B) The genes involved in casein synthesis. Data represent the mean and standard deviation (n = 6), and the asterisk indicates statistical difference (* p < 0.05) between the indicated columns, based on one-way analysis of variance and Duncan’s range test. IL-1β, interleukin-1β; IL-6, interleukin-6; IL-8, interleukin-8; TNF-α, tumor necrosis factor-α; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL6, chemokine (C-X-C motif) ligand 6; CSN1S1, αS1-casein; CSN2, β-casein; CSN3, κ-casein; RT-qPCR, reverse transcription quantitative real-time polymerase chain reaction; CON, control group; PGN, peptidoglycan group; LTA, lipoteichoic acid group; MIX, PGN + LTA group.